Feature Reference CSV is passed to cellranger count with the --feature-ref flag and declares the set of Feature Barcode reagents in use in the experiment.
For each unique Feature Barcode used, this file declares a feature name and identifier, the unique Feature Barcode sequence associated with this reagent, and a pattern indicating how to extract the Feature Barcode sequence from the read sequence.
Required Columns for Feature Reference CSV File
For Example: A valid Feature Reference CSV File for Antibody Capture barcode will be as shown in the table below
For more information on creating Feature Reference CSV File, follow the below link